Alfonso A, Louzao M C, Vieytes M R, Botana L M
Departamento de Farmacología, Facultad de Veterinaria, Lugo, Spain.
Toxicon. 1994 Dec;32(12):1593-8. doi: 10.1016/0041-0101(94)90318-2.
Paralytic shellfish poison (PSP) has historically been a problem for the shellfish industry. In order to prevent the marketing of contaminated seafood products, governments have implemented monitoring programs where standards of toxins are necessary. The stability of these standard toxins is very important. In this paper we analysed the stability of saxitoxin (STX) and neosaxitoxin in acidic solution and lyophilized samples. Individual toxins were determined in each sample using a high-performance liquid chromatographic procedure employing post-column oxidation of the toxins to form fluorescent derivatives. Our results demonstrate that STX is very stable in solution samples and could be adopted as a reference standard. This toxin can be kept in dilute acidic solutions for 18 months without loss of potency. However, neosaxitoxin is unstable, possibly due to transformation to other toxins.
麻痹性贝类毒素（PSP）一直以来都是贝类产业面临的一个问题。为防止受污染的海产品进入市场，各国政府已实施监测计划，其中毒素标准必不可少。这些标准毒素的稳定性非常重要。在本文中，我们分析了石房蛤毒素（STX）和新石房蛤毒素在酸性溶液和冻干样品中的稳定性。使用高效液相色谱法，通过柱后氧化毒素形成荧光衍生物，测定每个样品中的单一毒素。我们的结果表明，STX在溶液样品中非常稳定，可作为参考标准。这种毒素可以保存在稀酸性溶液中18个月而不会丧失效力。然而，新石房蛤毒素不稳定，可能是由于转化为其他毒素所致。
