Sandré C, Géniteau-Legendre M, Scherrmann J M, Quéro A M, Labarre C
Laboratoire de Virologie et Immunologie Expérimentales, Centre d'Etudes Pharmaceutiques, Châtenay-Malabry, France.
Ther Drug Monit. 1995 Feb;17(1):19-24. doi: 10.1097/00007691-199502000-00004.
The presence of digitoxin-like immunoreactive substances, whose nature is yet unknown, has been demonstrated in the umbilical cord blood. We selected six antidigitoxin monoclonal antibodies (MAb) having different specificity profiles concerning digitoxin analogs and steroid hormones. These antibodies were tested in a digitoxin radioimmunoassay (RIA). With the help of this technique, we measured the concentrations of apparent digitoxin in the cord blood drawn either at birth or in utero from mothers not undergoing any digitalis treatment. In the cord blood of newborns, the concentrations of apparent digitoxin, measured by the two MAbs that have the highest cross-reactions with dehydroepiandrosterone (DHEA) (123A23 and 145A41), were two or three times higher than with the other antibodies. In the fetal cord blood, where the concentration of DHEA is five to seven times lower than that observed at birth, these antibodies revealed a threefold lower concentration of apparent digitoxin than that observed in blood drawn at birth. Furthermore, MAbs that had similar specificities towards digitoxin analogs and steroids showed different measurements of digitoxin-like concentrations. These observations suggest that digitoxin-like immunoreactive compounds detected by the RIA may constitute a group of different molecules, one of which would be the DHEA.
在脐带血中已证实存在性质尚不清楚的洋地黄毒苷样免疫反应性物质。我们选择了六种针对洋地黄毒苷类似物和甾体激素具有不同特异性谱的抗洋地黄毒苷单克隆抗体（MAb）。这些抗体在洋地黄毒苷放射免疫分析（RIA）中进行了检测。借助这项技术，我们测量了从未接受任何洋地黄治疗的母亲在出生时或子宫内采集的脐带血中表观洋地黄毒苷的浓度。在新生儿的脐带血中，与脱氢表雄酮（DHEA）交叉反应最高的两种单克隆抗体（123A23和145A41）所测得的表观洋地黄毒苷浓度，比其他抗体测得的浓度高两到三倍。在胎儿脐带血中，DHEA的浓度比出生时观察到的浓度低五到七倍，这些抗体所显示的表观洋地黄毒苷浓度比出生时采集的血液中观察到的浓度低三倍。此外，对洋地黄毒苷类似物和甾体具有相似特异性的单克隆抗体对洋地黄毒苷样浓度的测量结果不同。这些观察结果表明，放射免疫分析检测到的洋地黄毒苷样免疫反应性化合物可能构成一组不同的分子，其中一种可能是DHEA。
