毒毛花苷（Na⁺，K⁺ - ATP酶的内源性抑制剂）与抗地高辛及抗洋地黄毒苷抗体的相互作用。

Interaction of cardiodigin, endogenous inhibitor of Na+,K+-ATPase, with antidigoxin and antidigitoxin antibodies.

作者信息

Fagoo M, Godfraind T

出版信息

Biochem Biophys Res Commun. 1985 Jun 14;129(2):553-9. doi: 10.1016/0006-291x(85)90187-1.

Abstract

The present study was undertaken to further characterize the immunoreactivity of cardiodigin, digitalis-like factor present in mammalian tissues. Guinea-pig heart extracts purified by reverse-phase low pressure and high pressure liquid chromatographies were analysed for their cross-reactivity with antidigitoxin and antidigoxin antibodies. The putative digitalis-like factor showed an affinity about 10 times higher for antidigoxin antibodies than for antidigitoxin antibodies. EC50 ratios (digoxin/digitoxin assay) determined at two purification steps were different from those of digoxin and digitoxin. These results show that cardiodigin presents molecular determinants recognizable by antidigoxin and antidigitoxin antibodies but that it is a chemical entity different from these well known cardioactive steroids.

摘要

本研究旨在进一步表征哺乳动物组织中存在的洋地黄毒苷样因子——强心苷的免疫反应性。对通过反相低压和高压液相色谱法纯化的豚鼠心脏提取物进行分析，以检测其与抗洋地黄毒苷抗体和抗地高辛抗体的交叉反应性。推测的洋地黄毒苷样因子与抗地高辛抗体的亲和力比对抗洋地黄毒苷抗体的亲和力高约10倍。在两个纯化步骤中测定的EC50比值（地高辛/洋地黄毒苷测定）与地高辛和洋地黄毒苷的不同。这些结果表明，强心苷具有可被抗地高辛和抗洋地黄毒苷抗体识别的分子决定簇，但它是一种与这些知名的心脏活性甾体不同的化学实体。