Hara K, Funakoshi M, Tsuda K, Kawarabata T
Institute of Biological Control, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Acta Virol. 1994 Oct;38(5):291-4.
The nuclear polyhedrosis viruses of Spodoptera exigua (SeNPV) and Autographa californica (AcNPV) produced plaques in a newly established cell line of the beet armyworm, Spodoptera exigua. Plaques were composed of infected cells containing many polyhedra and were visible without any staining procedure. Dose-response assays showed a direct correlation between the number of plaques and the inoculum size. Growth kinetic studies of the two viruses, using the developed plaque assay system, revealed that the release of extracellular viruses began 6 hrs post infection (p.i.) and the titer reached a plateau 48 hrs p.i. The sensitivity of this plaque assay system was 100 times greater for the heterologous AcNPV than for the homologous SeNPV.
甜菜夜蛾核型多角体病毒（SeNPV）和苜蓿银纹夜蛾核型多角体病毒（AcNPV）在新建立的甜菜夜蛾细胞系中形成了噬菌斑。噬菌斑由含有许多多角体的感染细胞组成，无需任何染色程序即可看见。剂量反应试验表明噬菌斑数量与接种量之间存在直接相关性。使用所建立的噬菌斑测定系统对这两种病毒进行的生长动力学研究显示，感染后6小时（p.i.）开始释放细胞外病毒，48小时p.i.时滴度达到平台期。该噬菌斑测定系统对异源AcNPV的敏感性比对同源SeNPV高100倍。
