Podgorskiĭ V S, Gavrilenko M N, Sumnevich V G, Zyrianova L F
Mikrobiol Z. 1995 Jan-Feb;57(1):19-24.
Joint effect of various concentrations of alcohol and saccharose in the medium as well as the influence of temperature are studied under conditions of batch cultivation of Saccharomyces cerevisiae. It has been found that saccharose added to the medium in the amount exceeding 1% is assimilated less efficiently. So, it is not expedient to exceed the above concentration in industrial nutrient media with 10-12 vol. % of ethanol. The biomass yield within 18-22 degrees C depends only on the alcohol amount in the medium. A further rise in temperature to 35 degrees C induces its inhibitory effect ever growing with the ethanol concentration in the medium. Mathematical modeling shows basic regularities of the growth process which permit predicting behaviour of the culture in the studied factorial space. Optimal values of saccharose concentrations and cultivation temperature for the most efficient cultivation of yeast on the ethanol-containing medium are determined.
在酿酒酵母分批培养条件下，研究了培养基中不同浓度酒精和蔗糖的联合作用以及温度的影响。已发现，添加到培养基中的蔗糖量超过1%时，其同化效率较低。因此，在含有10 - 12体积%乙醇的工业营养培养基中，超过上述浓度是不合适的。在18 - 22摄氏度范围内，生物量产量仅取决于培养基中的酒精含量。温度进一步升高至35摄氏度时，其抑制作用会随着培养基中乙醇浓度的增加而不断增强。数学建模显示了生长过程的基本规律，这些规律能够预测所研究因子空间中培养物的行为。确定了在含乙醇培养基上最有效地培养酵母时蔗糖浓度和培养温度的最佳值。
