用重组猴病毒40大肿瘤抗原免疫小鼠后诱导产生的淋巴因子检测。
Examination of lymphokines induced in mice following immunization with recombinant simian virus 40 large tumor antigen.
作者信息
Bright R K, Shearer M H, Kennedy R C
机构信息
Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas 78228, USA.
出版信息
Cancer Immunol Immunother. 1995 Mar;40(3):206-11. doi: 10.1007/BF01517353.
Baculovirus-derived recombinant simian virus 40 (SV40) large tumor antigen (T-Ag) was used to immunize BALB/c mice to examine the lymphokines produced following immunization. Specifically, we examined production of interleukin-2 (IL-2), IL-4, IL-5 and interferon gamma (IFN gamma) from immune lymphocytes cultured with decreasing concentrations of recombinant SV40 T-Ag. We identified elevated levels of IFN gamma and IL-2 by enzyme-linked immunosorbent assay and a murine CTLL-2 proliferation bioassay respectively. We were unable to detect either IL-4 or IL-5. These data indicate the previously reported tumor immunity induced by recombinant SV40 T-Ag immunization most likely reflects a TH1-like immune response based on the in vitro production of both IFN gamma and IL-2 by immune lymphocytes.
利用杆状病毒衍生的重组猿猴病毒40（SV40）大T抗原（T-Ag）免疫BALB/c小鼠，以检测免疫后产生的淋巴因子。具体而言，我们检测了用浓度逐渐降低的重组SV40 T-Ag培养的免疫淋巴细胞产生白细胞介素-2（IL-2）、IL-4、IL-5和干扰素γ（IFNγ）的情况。我们分别通过酶联免疫吸附测定和小鼠CTLL-2增殖生物测定法确定了IFNγ和IL-2水平升高。我们未能检测到IL-4或IL-5。这些数据表明，先前报道的重组SV40 T-Ag免疫诱导的肿瘤免疫很可能反映了一种基于免疫淋巴细胞在体外产生IFNγ和IL-2的类似TH1的免疫反应。
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