Coukell B, Moniakis J, Grinberg A
Department of Biology, York University, Ont., Canada.
FEBS Lett. 1995 Apr 10;362(3):342-6. doi: 10.1016/0014-5793(95)00272-b.
We have cloned a full-length cDNA from Dictyostelium discoideum which encodes a new Ca(2+)-binding protein. The deduced protein (termed CBP1) is composed of 156 amino acids and contains four consensus metal-ligating loop sequences found in helix-loop-helix motifs of many Ca(2+)-binding proteins. When expressed in bacteria as a GST fusion protein, CBP1 binds Ca2+ in a 45Ca2+ overlay assay. CBP1 exhibits little amino acid sequence homology with Dictyostelium calmodulin or calfumirin-1 (CAF-1) except in the putative Ca(2+)-binding regions. Moreover, unlike calmodulin and CAF-1 expression, CBP1 mRNA is expressed preferentially during the multicellular stages of development.
我们从盘基网柄菌中克隆了一个全长cDNA，它编码一种新的钙结合蛋白。推导的蛋白质（称为CBP1）由156个氨基酸组成，并且包含在许多钙结合蛋白的螺旋-环-螺旋基序中发现的四个共有金属连接环序列。当作为GST融合蛋白在细菌中表达时，CBP1在45Ca2+覆盖分析中结合Ca2+。除了在假定的钙结合区域外，CBP1与盘基网柄菌钙调蛋白或钙调素-1（CAF-1）几乎没有氨基酸序列同源性。此外，与钙调蛋白和CAF-1的表达不同，CBP1 mRNA在发育的多细胞阶段优先表达。
