Benka M L, Lee M, Wang G R, Buckman S, Burlacu A, Cole L, DePina A, Dias P, Granger A, Grant B
Marine Biological Laboratory, Woods Hole, MA, USA.
FEBS Lett. 1995 Apr 17;363(1-2):49-52. doi: 10.1016/0014-5793(95)00278-h.
The thrombin receptor is a G protein-coupled receptor, but the G proteins functionally coupled to this receptor in human platelets are not yet definitively identified. Thrombin stimulation of platelets leads to phospholipase C-mediated increases in intracellular calcium, and previous studies have suggested that the thrombin receptor is coupled to members of the Gq family. We now demonstrate direct GTPase activation by thrombin receptor activation peptide (TRAP) in human platelet membranes, and specific inhibition of TRAP-activated GTPase by antibodies to Gq. These data demonstrate functional coupling of the thrombin receptor to a member of the Gq family.
凝血酶受体是一种G蛋白偶联受体，但在人类血小板中与该受体功能偶联的G蛋白尚未得到明确鉴定。凝血酶对血小板的刺激会导致磷脂酶C介导的细胞内钙增加，先前的研究表明凝血酶受体与Gq家族成员偶联。我们现在证明了凝血酶受体激活肽（TRAP）在人血小板膜中直接激活GTP酶，以及针对Gq的抗体对TRAP激活的GTP酶的特异性抑制。这些数据证明了凝血酶受体与Gq家族成员的功能偶联。
