Kawamura M, Tajima S, Azuma N, Katsura H, Akiyama K
Department of Ophthalmology, Keiyu General Hospital, Kangawa, Japan.
Graefes Arch Clin Exp Ophthalmol. 1995 Feb;233(2):58-62. doi: 10.1007/BF00241472.
Since Brockhurst reported the connection between uveal effusion in nanophthalmic eyes and their scleral alterations and treated them with vortex vein decompression or sclerectomy, many observers have found abnormal accumulation of glycosaminoglycans (GAGs) in nanophthalmic sclera. These GAG abnormalities were thought to effect the collagen changes, though it was not clear which GAGs were changed.
GAGs were isolated and their contents were determined in scleral specimens from three nanophthalmic patients and five age-matched controls, using electrophoresis and the cetylpyridinium method.
Hyaluronic acid, dermatan sulfate and chondroitin sulfate were the major GAGs in both nanophthalmic and control samples. Nanophthalmic sclera showed 2.4-fold, 10-fold and 5.5-fold increases in hyaluronic acid, dermatan sulfate and chondroitin sulfate, respectively, compared with the controls.
The results suggest that increased levels of GAGs, particularly of dermatan sulfate and chondroitin sulfate may contribute to the abnormalities of collagen fibrillogenesis and be closely involved with the pathogenesis of nanophthalmos.
自从布罗克赫斯特报道了小眼球眼中葡萄膜渗漏与其巩膜改变之间的联系，并采用涡静脉减压术或巩膜切除术进行治疗以来，许多观察者发现小眼球巩膜中存在糖胺聚糖（GAGs）异常积聚。尽管尚不清楚哪些GAGs发生了变化，但这些GAGs异常被认为会影响胶原蛋白的变化。
使用电泳法和十六烷基吡啶法，从三名小眼球患者和五名年龄匹配的对照者的巩膜标本中分离出GAGs并测定其含量。
透明质酸、硫酸皮肤素和硫酸软骨素是小眼球样本和对照样本中的主要GAGs。与对照相比，小眼球巩膜中的透明质酸、硫酸皮肤素和硫酸软骨素分别增加了2.4倍、10倍和5.5倍。
结果表明，GAGs水平升高，尤其是硫酸皮肤素和硫酸软骨素水平升高，可能导致胶原纤维形成异常，并与小眼球病的发病机制密切相关。
