Coates A A, Menino A R
Department of Animal Sciences, Oregon State University, Corvallis 97331-6702.
J Anim Sci. 1994 Nov;72(11):2936-42. doi: 10.2527/1994.72112936x.
Two experiments were conducted to evaluate factors affecting zona pellucida (ZP) solubility in bovine embryos during hatching in vitro. In Exp. 1, the relationship between blastocoelic expansion and ZP solubility was determined. Day-6 embryos (n = 42) with good or excellent quality grades were cultured (Cultured embryos), whereas embryos of fair quality (Not-cultured embryos) were immediately placed in .2% SDS, and the time required for complete dissolution of the ZP (ZPDT) was determined. For Cultured embryos, ZPDT was determined after 192 h of in vitro development. Zona pellucida dissolution time was greater (P < .05) in Not-cultured embryos than in Cultured embryos and negatively correlated (P < .01) with changes in embryonic surface area. In Exp. 2, the effects of suppressing plasminogen activator (PA) activity and blastocoelic expansion on hatching and ZP solubility were examined. Day-6 embryos (n = 99) were cultured in medium containing 0, 10, or 100 IU/mL of human PA inhibitor-2 (PAI-2) and incubated for 24 to 44 h in medium containing 0, .1, or .5 nM ouabain. Percentages of embryos hatching were not different (P > .05) among 0, 10, and 100 IU/mL of PAI-2; however, more (P < .05) embryos hatched after exposure to 0 and .1 nM ouabain than to .5 nM ouabain. Embryonic PA activity was suppressed (P < .05) by PAI-2, whereas exposure to ouabain did not affect (P > .05) PA activity. Mean ZPDT did not differ (P > .05) following culture in medium containing PAI-2 or exposure to ouabain.(ABSTRACT TRUNCATED AT 250 WORDS)
进行了两项实验以评估体外孵化期间影响牛胚胎透明带（ZP）溶解性的因素。在实验1中，确定了囊胚腔扩张与ZP溶解性之间的关系。将质量等级为良好或优秀的第6天胚胎（n = 42）进行培养（培养胚胎），而质量一般的胚胎（未培养胚胎）立即置于0.2%十二烷基硫酸钠中，并测定ZP完全溶解所需的时间（ZPDT）。对于培养胚胎，在体外发育192小时后测定ZPDT。未培养胚胎的透明带溶解时间比培养胚胎更长（P < 0.05），且与胚胎表面积变化呈负相关（P < 0.01）。在实验2中，研究了抑制纤溶酶原激活剂（PA）活性和囊胚腔扩张对孵化和ZP溶解性的影响。将第6天胚胎（n = 99）在含有0、10或100 IU/mL人PA抑制剂-2（PAI-2）的培养基中培养，并在含有0、0.1或0.5 nM哇巴因的培养基中孵育24至44小时。在0、10和100 IU/mL的PAI-2之间，胚胎孵化率没有差异（P > 0.05）；然而，暴露于0和0.1 nM哇巴因后孵化的胚胎比暴露于0.5 nM哇巴因的更多（P < 0.05）。PAI-2抑制了胚胎PA活性（P < 0.05），而暴露于哇巴因对PA活性没有影响（P > 0.05）。在含有PAI-2的培养基中培养或暴露于哇巴因后，平均ZPDT没有差异（P > 0.05）。（摘要截断于250字）
