Miyoshi-Akiyama T, Abe A, Kato H, Kawahara K, Narimatsu H, Uchiyama T
Department of Microbiology and Immunology, Tokyo Women's Medical College, Japan.
J Immunol. 1995 May 15;154(10):5228-34.
Previously, we found a novel bacterial superantigen from Yersinia pseudotuberculosis, designated Y. pseudotuberculosis-derived mitogen (YPM). In the present study, we analyzed the DNA sequence of the gene encoding YPM. The YPM gene was cloned into a plasmid vector pMW119 and expressed in Escherichia coli DH10B. Like the native YPM, the cloned YPM required the expression of MHC class II molecules on accessory cells in the induction of IL-2 production by human T cells. TCR-V beta repertoire of human T cells reactive with the cloned YPM was V beta 3, V beta 9, V beta 13.1, and V beta 13.2. This repertoire is the same as that of T cells reactive with the native YPM. These results indicate that the cloned YPM expressed in E. coli is identical to the native YPM. Sequencing of the YPM gene revealed that the gene contained an open reading frame of 456 base pairs encoding a precursor form of 151 amino acid residues with m.w. 16,679 that is processed into a mature form of 131 amino acid residues with m.w. 14,529. Homology analysis revealed that the homology of amino acid sequence is quite low among YPM and other well known bacterial superantigens. We designated the gene encoding YPM as ypm.
此前，我们从假结核耶尔森菌中发现了一种新型细菌超抗原，命名为假结核耶尔森菌衍生丝裂原（YPM）。在本研究中，我们分析了编码YPM的基因的DNA序列。将YPM基因克隆到质粒载体pMW119中，并在大肠杆菌DH10B中表达。与天然YPM一样，克隆的YPM在诱导人T细胞产生IL-2时需要辅助细胞上MHC II类分子的表达。与克隆的YPM反应的人T细胞的TCR-Vβ谱系为Vβ3、Vβ9、Vβ13.1和Vβ13.2。该谱系与与天然YPM反应的T细胞的谱系相同。这些结果表明在大肠杆菌中表达的克隆YPM与天然YPM相同。YPM基因测序显示该基因包含一个456个碱基对的开放阅读框，编码一种151个氨基酸残基的前体形式，分子量为16,679，加工后成为一种131个氨基酸残基的成熟形式，分子量为14,529。同源性分析表明，YPM与其他知名细菌超抗原之间的氨基酸序列同源性相当低。我们将编码YPM的基因命名为ypm。
