Haq T A, Mason H S, Clements J D, Arntzen C J
Plant Biotechnology Program, Albert B. Alkek Institute of Biosciences and Technology, Texas A&M University, Houston 77030, USA.
Science. 1995 May 5;268(5211):714-6. doi: 10.1126/science.7732379.
The binding subunit of Escherichia coli heat-labile enterotoxin (LT-B) is a highly active oral immunogen. Transgenic tobacco and potato plants were made with the use of genes encoding LT-B or an LT-B fusion protein with a microsomal retention sequence. The plants expressed the foreign peptides, both of which formed oligomers that bound the natural ligand. Mice immunized by gavage produced serum and gut mucosal anti-LT-B immunoglobulins that neutralized the enterotoxin in cell protection assays. Feeding mice fresh transgenic potato tubers also caused oral immunization.
大肠杆菌不耐热肠毒素（LT-B）的结合亚基是一种高效的口服免疫原。利用编码LT-B或带有微粒体保留序列的LT-B融合蛋白的基因培育出了转基因烟草和马铃薯植株。这些植株表达了外源肽，二者均形成了能结合天然配体的寡聚体。通过灌胃免疫的小鼠产生了血清和肠道黏膜抗LT-B免疫球蛋白，这些免疫球蛋白在细胞保护试验中可中和肠毒素。给小鼠喂食新鲜的转基因马铃薯块茎也能引发口服免疫。
