McCullagh K J, Bonen A
Department of Anatomy and Physiology, Trinity College, Dublin, Ireland.
Am J Physiol. 1995 Apr;268(4 Pt 2):R884-8. doi: 10.1152/ajpregu.1995.268.4.R884.
The purpose of this study was to investigate the effect of the neural regulation of contractile activity on lactate transport in skeletal muscle. Contractile activity of the rat soleus muscle was abolished by denervating the hindlimb muscles in one leg (3 days) while the sham-operated contralateral hindlimb muscles served as a control. Three days after surgery, lactate transport into the soleus muscle was measured in vitro, using incubated soleus muscle strips. Lactate uptake by the denervated soleus muscle was reduced compared with control (P < 0.05). The diffusive component of lactate transport was unaltered by denervation (P > 0.05). These results translated into a reduction in lactate carrier-mediated transport capacity (-68%) in the denervated soleus (P < 0.05). These studies indicate that loss of contractile activity results in a decrement of lactate transport, which is probably due to a reduction in the number of lactate carriers in the sarcolemma. Our results suggest that the inherent activity of the muscle is important in maintaining the lactate transport system.
本研究的目的是探讨收缩活动的神经调节对骨骼肌中乳酸转运的影响。通过使一侧后肢肌肉去神经支配（3天）来消除大鼠比目鱼肌的收缩活动，而对侧假手术的后肢肌肉作为对照。手术后3天，使用孵育的比目鱼肌条在体外测量乳酸向比目鱼肌的转运。与对照相比，去神经支配的比目鱼肌对乳酸的摄取减少（P < 0.05）。去神经支配对乳酸转运的扩散成分没有影响（P > 0.05）。这些结果转化为去神经支配的比目鱼肌中乳酸载体介导的转运能力降低（-68%）（P < 0.05）。这些研究表明，收缩活动的丧失导致乳酸转运减少，这可能是由于肌膜中乳酸载体数量减少所致。我们的结果表明，肌肉的固有活动对维持乳酸转运系统很重要。
