Yoshimura S, Sakamoto S, Kudo H, Suzuki S, Sassa S, Matsubara M, Nagasawa H
Medical Research Institute, Tokyo Medical and Dental University, Japan.
Anticancer Res. 1995 Jan-Feb;15(1):61-5.
The suppressive effects of danazol, an isoxazol derivative of a synthetic steroid 17 alpha-ethinyltestosterone, on cellular viability and DNA synthesis in 7,12-dimethylbenz(a)anthracene induced mammary tumours were investigated in adult female rats by enzyme assays and immunohistochemistry with bromodeoxyuridine (BrdU). Rats treated with danazol for 30 days showed a decrease of plasma levels of luteinizing hormone and estradiol associated with a dysfunction in hypothalamo-hypophysial-gonadal axis, resulting in lower activities in succinate dehydrogenase and thymidine kinase, and a reduction of BrdU-immunoreactive cells in mammary tumours compared with the control, i.e., decreases of viability and pyrimidine nucleotide synthesis in tumour cells in danazol-treated rats.
通过酶分析以及使用溴脱氧尿苷（BrdU）的免疫组织化学方法，研究了合成类固醇17α-乙炔睾酮的异恶唑衍生物达那唑对成年雌性大鼠7,12-二甲基苯并（a）蒽诱导的乳腺肿瘤细胞活力和DNA合成的抑制作用。用达那唑治疗30天的大鼠，其促黄体生成素和雌二醇的血浆水平降低，伴有下丘脑 - 垂体 - 性腺轴功能障碍，导致琥珀酸脱氢酶和胸苷激酶活性降低，与对照组相比，乳腺肿瘤中BrdU免疫反应性细胞减少，即达那唑治疗的大鼠肿瘤细胞活力和嘧啶核苷酸合成降低。
