Smith J B, Yang Z, Lin P, Zaidi Z, Abbasi A, Russell P
Department of Medicinal Chemistry, Purdue University, West Lafayette, IN 47907, USA.
Biochem J. 1995 Apr 15;307 ( Pt 2)(Pt 2):407-10. doi: 10.1042/bj3070407.
The complete sequence of human gamma s-crystallin has been determined and confirmed using a combination of MS methods, peptide sequencing and cDNA sequencing. Regions 21-35 and 102-107, which were previously assumed to be the same as the bovine sequence, differ from the bovine sequence at residues 22, 28, 31 and 104. An additional six residues were also found to be different from the original sequence determined for Pakastani lenses. Whether these differences represent errors in the original sequence or two different sequences among human lens crystallins is not yet known.
已通过质谱方法、肽测序和 cDNA 测序相结合的方式确定并证实了人类γ - s - 晶状体蛋白的完整序列。先前认为与牛序列相同的第 21 - 35 区域和 102 - 107 区域，在第 22、28、31 和 104 位残基处与牛序列不同。还发现另外六个残基与为巴基斯坦晶状体确定的原始序列不同。这些差异是代表原始序列中的错误，还是人类晶状体蛋白中的两种不同序列，目前尚不清楚。
