Kawashima H, Strobel H W
Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston 77225, USA.
Biochem Biophys Res Commun. 1995 Apr 17;209(2):535-40. doi: 10.1006/bbrc.1995.1534.
Two cDNA clones designated 2d-29 and 2d-35 encoding a novel P450 belonging to the P450 2D subfamily were isolated from control and imipramine-treated rat brain libraries, respectively. cDNA 2d-29 and 2d-35 contained 1786 and 2754 bp nucleotides each. Although their 5'- and 3'-untranslated sequences were different from each other, their open reading frames were identical, encoding a protein which had 5 substitutions of amino acids in the C-terminal region compared with P450 2D4. Northern blot analysis showed that the expression levels of 2d-29 and 2d-35 in the brain were lower than that of P450 2D4 in the liver. However, it is noteworthy that the expression of 2d-35 was not detected in the liver but in the brain. These results suggest that a novel P450 is expressed in the brain under different regulation.
分别从对照大鼠和经丙咪嗪处理的大鼠脑文库中分离出两个cDNA克隆，命名为2d - 29和2d - 35，它们编码一种属于P450 2D亚家族的新型P450。cDNA 2d - 29和2d - 35各自含有1786和2754个碱基对核苷酸。尽管它们的5'和3'非翻译序列彼此不同，但它们的开放阅读框是相同的，编码一种与P450 2D4相比在C末端区域有5个氨基酸取代的蛋白质。Northern印迹分析表明，2d - 29和2d - 35在脑中的表达水平低于P450 2D4在肝脏中的表达水平。然而，值得注意的是，在肝脏中未检测到2d - 35的表达，而在脑中检测到了。这些结果表明，一种新型P450在脑中以不同的调控方式表达。
