Ventura F, Ambrosio S, Bartrons R, el-Maghrabi M R, Lange A J, Pilkis S J
Unitat de Bioquimica, Universitat de Barcelona, Spain.
Biochem Biophys Res Commun. 1995 Apr 26;209(3):1140-8. doi: 10.1006/bbrc.1995.1616.
A cDNA encoding the catalytic core of a novel brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase isoenzyme was isolated from a lambda gt10 bovine brain library. This brain cDNA begins and ends in an open reading frame encoding a peptide of 476 amino acids. This peptide contains both the catalytic kinase and bisphosphatase domains and has an overall 65% and 67% indentity with the bovine heart and liver isozymes, respectively, whereas the NH2 and COOH-termini are divergent. An active catalytic core brain bifunctional enzyme was expressed in E. coli using a T7 RNA polymerase-based expression system. These results support the presence of a distinct gene coding for the protein in bovine brain.
从λgt10牛脑文库中分离出一个编码新型脑6-磷酸果糖-2-激酶/果糖-2,6-二磷酸酶同工酶催化核心的cDNA。这个脑cDNA在一个编码476个氨基酸肽段的开放阅读框中起始和终止。该肽段同时包含催化激酶和双磷酸酶结构域，与牛心和肝同工酶的总体一致性分别为65%和67%，而其氨基端和羧基端则有所不同。使用基于T7 RNA聚合酶的表达系统在大肠杆菌中表达了具有活性催化核心的脑双功能酶。这些结果支持在牛脑中存在一个编码该蛋白质的独特基因。
