Ohuchi H, Nakagawa T, Yamauchi M, Ohata T, Yoshioka H, Kuwana T, Mima T, Mikawa T, Nohno T, Noji S
Department of Biological Science and Technology, Faculty of Engineering, University of Tokushima, Japan.
Biochem Biophys Res Commun. 1995 Apr 26;209(3):809-16. doi: 10.1006/bbrc.1995.1572.
To elucidate what initiates formation of the limb, we have attempted to induce an additional limb from the flank of the chick embryo by infecting retrovirus or implanting cells. We report here that an additional limb can be formed from the flank when we implant fibroblast growth factor 4 (Fgf4)-expressing cells into the lateral plate mesoderm at the pre-limb bud stage. In a newly formed limb bud, expressions of both Sonic hedgehog and chick Fgf4, which are authentic morphogenetic signals from the zone of polarizing activity and the apical ectodermal ridge, respectively, are induced by the implanted cells. Thus, it is concluded that the competence for limb development is present along the flank of the chick embryo and that FGF4 applied ectopically at the pre-limb bud stage can alter the developmental fate of flank cells to become limb cells. The present experimental system will contribute to a further elucidation on how the limb is formed.
为了阐明是什么启动了肢体的形成，我们尝试通过感染逆转录病毒或植入细胞，从鸡胚的侧翼诱导出额外的肢体。我们在此报告，当我们在肢体芽前期将表达成纤维细胞生长因子4（Fgf4）的细胞植入侧板中胚层时，可以从侧翼形成额外的肢体。在新形成的肢体芽中，植入的细胞诱导了音猬因子和鸡Fgf4的表达，它们分别是来自极化活性区和顶端外胚层嵴的真正形态发生信号。因此，可以得出结论，鸡胚侧翼存在肢体发育的能力，并且在肢体芽前期异位应用FGF4可以改变侧翼细胞的发育命运，使其成为肢体细胞。本实验系统将有助于进一步阐明肢体是如何形成的。
