大鼠血管紧张素II 1A型受体基因糖皮质激素反应元件的特征分析

Characterization of glucocorticoid response element of rat angiotensin II type 1A receptor gene.

作者信息

Murasawa S, Matsubara H, Kanasaki M, Kijima K, Maruyama K, Nio Y, Okubo N, Tsukaguchi H, Mori Y, Inada M

机构信息

Second Department of Internal Medicine, Kansai Medical University, Osaka, Japan.

出版信息

Biochem Biophys Res Commun. 1995 Apr 26;209(3):833-40. doi: 10.1006/bbrc.1995.1575.

DOI:10.1006/bbrc.1995.1575

PMID:7733975

Abstract

The responsiveness of the rat angiotensin II type 1a and type 1b receptor (AT1a-R and AT1b-R) genes to glucocorticoid was examined in rat vascular smooth muscle cells (VSMCs) and the glucocorticoid response element (GRE) of the AT1a-R gene was characterized. Glucocorticoid induced an increase in AT1a-R mRNA levels, whereas AT1b-R mRNA levels were unaffected. The nuclear run-off assay indicated that the transcription of the AT1a-R gene, but not that of the AT1b-R gene, was increased by glucocorticoid. The mRNA stability of AT1a-R was unchanged by glucocorticoid. Promoter/chrolamphenicol acetyltransferase reporter analysis demonstrated that the 5'-flanking region of the AT1a-R gene was functional in rat VSMCs and established that the GRE motif between -770 to -756 could confer glucocorticoid responsiveness on the AT1a-R gene.

摘要

在大鼠血管平滑肌细胞（VSMC）中检测了大鼠血管紧张素II 1a型和1b型受体（AT1a-R和AT1b-R）基因对糖皮质激素的反应性，并对AT1a-R基因的糖皮质激素反应元件（GRE）进行了表征。糖皮质激素诱导AT1a-R mRNA水平升高，而AT1b-R mRNA水平未受影响。核转录分析表明，糖皮质激素增加了AT1a-R基因的转录，但未增加AT1b-R基因的转录。糖皮质激素对AT1a-R的mRNA稳定性没有影响。启动子/氯霉素乙酰转移酶报告基因分析表明，AT1a-R基因的5'侧翼区域在大鼠VSMC中具有功能，并确定-770至-756之间的GRE基序可赋予AT1a-R基因糖皮质激素反应性。