Liu C C, Walsh C M, Young J D
Laboratory of Molecular Immunology and Cell Biology, Rockefeller University, New York, NY 10021, USA.
Immunol Today. 1995 Apr;16(4):194-201. doi: 10.1016/0167-5699(95)80121-9.
Perforin is a cytolytic mediator produced by killer lymphocytes, and is stored in and released by cytoplasmic granules. The protein is partially homologous to the terminal components of the membrane attack complex of complement and produces pores of up to 20 nm in diameter on target membranes. Its genomic and protein structures have recently been unraveled, and its function elucidated through the availability of genetically engineered, perforin-deficient mice. Here Chau-Ching Liu, Craig M. Walsh and John Ding-E Young briefly outline certain biochemical and molecular features of perforin, and discuss the still-evolving issues concerning the relevance of perforin and Fas in cell killing.
穿孔素是一种由杀伤性淋巴细胞产生的细胞溶解介质，储存于细胞质颗粒中并从中释放。该蛋白质与补体膜攻击复合物的末端成分部分同源，可在靶细胞膜上形成直径达20纳米的孔。其基因组和蛋白质结构最近已被阐明，通过基因工程构建的穿孔素缺陷小鼠，其功能也得以明确。本文中，刘兆清、克雷格·M·沃尔什和丁一扬简要概述了穿孔素的某些生化和分子特征，并讨论了关于穿孔素和Fas在细胞杀伤中的相关性这一仍在不断发展的问题。
