Kishi M, Tokunaga K, Zheng Y H, Bahmani M K, Kakinuma M, Nonoyama M, Lai P K, Ikuta K
Section of Serology, Hokkaido University, Sapporo, Japan.
AIDS Res Hum Retroviruses. 1995 Jan;11(1):45-53. doi: 10.1089/aid.1995.11.45.
The partially CD4-expressing T cell clone, Vpr-1, which carries a latent vpr-defective HIV-1 genome and expresses HIV-1 Nef protein only, was permissive to superinfection by HIV-1. Superinfection of Vpr-1 with vif- or vpu-defective mutants, which were noncytopathic, reactivated the vpr-defective virus and led to homologous recombination and cytopathogenesis. The data provide an experimental model for homologous recombination being an important mechanism whereby HIV-1 acquires genetic heterogeneity, and when occurring among defective virus in vivo bestows novel biological activities and virulence.
部分表达CD4的T细胞克隆Vpr-1携带潜伏性vpr缺陷型HIV-1基因组且仅表达HIV-1 Nef蛋白，它对HIV-1的超感染具有易感性。用无细胞病变作用的vif或vpu缺陷型突变体对Vpr-1进行超感染，可重新激活vpr缺陷型病毒并导致同源重组和细胞病变。这些数据提供了一个实验模型，表明同源重组是HIV-1获得遗传异质性的重要机制，并且当在体内缺陷病毒之间发生时会赋予新的生物学活性和毒力。
