大鼠肝脏甘氨酸甲基转移酶的结晶及初步X射线衍射研究

Crystallization and preliminary X-ray diffraction studies of glycine methyltransferase from rat liver.

作者信息

Fu Z, Takusagawa F, Konishi K, Takata Y, Fujioka M

机构信息

Department of Chemistry, University of Kansas, Lawrence 66045-0045, USA.

出版信息

J Struct Biol. 1994 Nov-Dec;113(3):247-9. doi: 10.1006/jsbi.1994.1059.

DOI:10.1006/jsbi.1994.1059

PMID:7734248

Abstract

Glycine methyltransferase from rat liver is a tetrameric enzyme with 292 amino acid residues in each identical subunit and catalyzes the AdoMet-dependent methylation of glycine to form sarcosine. The enzyme was crystallized by the hanging drop vapor diffusion method, using polyethylene glycol 4000 as a precipitant. The crystal belongs to the orthorhombic space group P2(1)2(1)2, with unit cell dimensions of a = 86.4, b = 175.7, c = 45.5 A and with two subunits in the asymmetric unit. The crystals diffract beyond 2.4 A resolution, and a set of 2.4 A resolution data were measured.

摘要

大鼠肝脏中的甘氨酸甲基转移酶是一种四聚体酶，每个相同亚基含有292个氨基酸残基，催化依赖于腺苷甲硫氨酸的甘氨酸甲基化反应生成肌氨酸。该酶采用悬滴气相扩散法进行结晶，使用聚乙二醇4000作为沉淀剂。晶体属于正交晶系空间群P2(1)2(1)2，晶胞参数为a = 86.4，b = 175.7，c = 45.5 Å，不对称单元中有两个亚基。这些晶体的衍射分辨率超过2.4 Å，并测量了一组2.4 Å分辨率的数据。