Lomnitski L, Sklan D, Grossman S
Dept of Life Sciences, Bar-Ilan University, Ramat Gan, Israel.
Biochim Biophys Acta. 1995 Apr 6;1255(3):351-9. doi: 10.1016/0005-2760(94)00239-u.
Lipoxygenase (LOX) activity in epidermis and dermis was distributed among microsomal and cytosolic fractions. The main products of polyunsaturated fatty acid metabolism were 12-hydroperoxy-cis-5,8,14, trans-10-eicosatetraenoic acid (12-HPETE), 15-hydroperoxy-cis-5,8,11, trans-13-eicosatetraenoic acid (15-HPETE) and 13-hydroxy-cis-9, trans-11-octadecadienoic acid (13-HOD). Enzyme activities were isolated from rat dermis and epidermis by ammonium sulphate precipitation, hydrophobic chromatography and gel filtration. In the dermis, activity was found at a molecular mass of 68 kDa, a pI of 4.6 and a Km of 50 microM. This activity was inhibited by known LOX inhibitors. The main reaction products indicated that this was 15-LOX. In the epidermis, activity was found in a fraction with a molecular mass of 68 kDa, a pI of 4.6 and a Km of 80 microM. Activity was inhibited by known LOX inhibitors whereas the reaction products indicated that this was 12-LOX. LOX activity in rat skin may involve one enzyme with dual regional specificities or may comprise two different enzymes.
表皮和真皮中的脂氧合酶（LOX）活性分布于微粒体和胞质部分。多不饱和脂肪酸代谢的主要产物为12-氢过氧-顺式-5,8,14，反式-10-二十碳四烯酸（12-HPETE）、15-氢过氧-顺式-5,8,11，反式-13-二十碳四烯酸（15-HPETE）和13-羟基-顺式-9，反式-11-十八碳二烯酸（13-HOD）。通过硫酸铵沉淀、疏水色谱和凝胶过滤从大鼠真皮和表皮中分离出酶活性。在真皮中，发现活性存在于分子量为68 kDa、pI为4.6、Km为50 μM的部分。该活性被已知的LOX抑制剂抑制。主要反应产物表明这是15-LOX。在表皮中，活性存在于分子量为68 kDa、pI为4.6、Km为80 μM的部分。活性被已知的LOX抑制剂抑制，而反应产物表明这是12-LOX。大鼠皮肤中的LOX活性可能涉及一种具有双重区域特异性的酶，也可能由两种不同的酶组成。
