Ohno H, Nakanishi E, Takamatsu T
Biotechnol Bioeng. 1976 Jun;18(6):847-64. doi: 10.1002/bit.260180607.
This work is concerned with the optimization study of the semibatch fermentation by which an amino acid is produced. The particular fermentation studied is the synthesis of lysine by the auxotrophic mutant. Applying Green's theorem to the maximization problem was proposed, and it succeeded in determining the feed rate of the substrate that maximized the production rate of the desired product.
这项工作涉及用于生产氨基酸的半间歇发酵的优化研究。所研究的特定发酵是营养缺陷型突变体合成赖氨酸。有人提出将格林定理应用于最大化问题，并且成功确定了使所需产物生产率最大化的底物进料速率。
