用4-（N-氯甲酰甲基-N-甲基）氨基-7-N，N-二甲基氨基磺酰基-2,1,3-苯并恶二唑（DBD-COCl）衍生化的花生四烯酸乙醇酰胺（花生四烯酸酰胺）及其类似物的高效液相色谱法和荧光检测
High-performance liquid chromatography and fluorometric detection of arachidonylethanolamide (anandamide) and its analogues, derivatized with 4-(N-chloroformylmethyl-N-methyl)amino-7-N,N-dimethylaminosulp honyl-2,1 ,3- benzoxadiazole (DBD-COCl).
作者信息
Koga D, Santa T, Hagiwara K, Imai K, Takizawa H, Nagano T, Hirobe M, Ogawa M, Sato T, Inoue K
机构信息
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
出版信息
Biomed Chromatogr. 1995 Jan-Feb;9(1):56-7. doi: 10.1002/bmc.1130090112.
The endogeneous ligand for the cannabinoid receptor, arachidonylethanolamide (anandamide) and its analogues, oleinylethanolamide, palmitylethanolamide and eicosapentaenoylethanolamide, were derivatized with a fluorogenic reagent, 4-(N-chloroformylmethyl-N-methyl)amino-7-N,N-dimethylaminsulpho ny1-2,1,3- benzoxadiazole (DBD-COCl). They were separated on a reversed phase HPLC with a mobile phase of acetonitrile:water. The fluorometric detection of the derivatives was made at 560 nm with excitation at 450 nm and the detection limits for anandamide was 20 fmol on column. The structures of DBD-CO-ethanolamides were confirmed by liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (LC-APCI-MS).
大麻素受体的内源性配体花生四烯酸乙醇胺（阿南达胺）及其类似物油酰乙醇胺、棕榈酰乙醇胺和二十碳五烯酸乙醇胺，用荧光试剂4-(N-氯甲酰甲基-N-甲基)氨基-7-N,N-二甲基氨基磺酰基-2,1,3-苯并恶二唑（DBD-COCl）进行衍生化。它们在以乙腈:水为流动相的反相高效液相色谱上进行分离。衍生物的荧光检测在560 nm处进行，激发波长为450 nm，柱上阿南达胺的检测限为20 fmol。通过液相色谱-大气压化学电离-质谱（LC-APCI-MS）确认了DBD-CO-乙醇酰胺的结构。
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