Daniotti J L, Allende M L, Weinberg E S, Allende J E
Departamento de Bioquímica, Facultad de Medicina, Universidad de Chile, Santiago.
Cell Mol Biol Res. 1994;40(5-6):431-9.
cDNA clones coding for the alpha and beta subunits of protein kinase 2 (CK2) in zebrafish (Danio rerio) have been isolated. Sequencing of the cDNA clones has demonstrated that one contains the complete coding sequence for the beta subunit of CK2 while the alpha clone is truncated and lacks 183 nucleotides of the 5' coding region. Comparison of the deduced amino acid sequences shows an extremely high degree of evolutionary sequence conservation of these two proteins. Northern analysis of the mRNAs coding for the alpha subunit indicates that this messenger is present in 1 h embryos as a 3.6 Kb and a 1.9 Kb species, both of which decrease in 24-h embryos. In the case of beta, the major mRNA species of approximately 1.7 Kb maintains its level during the period of embryogenesis studied. In situ hybridization of early embryos, using antisense RNAs against alpha and beta mRNAs demonstrates temporal and tissue specific expression patterns. The alpha mRNA decreases after blastula, when it is evenly distributed. The beta mRNA is maintained at high levels between 4 and 24 h of development, showing in 18 h embryos a higher concentration in the developing neural tube and in the embryonic optic and otic vesicles.
编码斑马鱼（Danio rerio）蛋白激酶2（CK2）α和β亚基的cDNA克隆已被分离出来。对这些cDNA克隆进行测序表明，其中一个包含CK2β亚基的完整编码序列，而α亚基克隆是截短的，缺少5'编码区的183个核苷酸。推导的氨基酸序列比较显示这两种蛋白质在进化上具有极高的序列保守性。对编码α亚基的mRNA进行的Northern分析表明，这种信使RNA在1小时胚胎中以3.6 kb和1.9 kb的形式存在，在24小时胚胎中这两种形式均减少。对于β亚基，在研究的胚胎发育期间，约1.7 kb的主要mRNA种类保持其水平。使用针对α和β mRNA的反义RNA对早期胚胎进行原位杂交，显示出时间和组织特异性表达模式。α mRNA在囊胚期后减少，此时它均匀分布。β mRNA在发育的4至24小时内保持高水平，在18小时胚胎中显示在发育中的神经管以及胚胎视泡和听泡中浓度较高。
