Roets E, Adams E, Muriithi I G, Hoogmartens J
Laboratorium voor Farmaceutische Chemie en Analyse van Geneesmiddelen, Faculteit Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, Belgium.
J Chromatogr A. 1995 Apr 7;696(1):131-8. doi: 10.1016/0021-9673(94)01272-g.
The determination of the relative amounts of the B and C components of neomycin sulphate by thin-layer chromatography using silica gel plates from Whatman as the stationary phase is described. The mobile phase consisted of methanol-20% (m/v) sodium chloride solution (15:85). Fluorescence detection was performed after derivatization with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole. The influence of different parameters on the separation was investigated. A number of commercial samples was analysed using this method and the results were compared with results obtained with ion-exchange chromatography and ninhydrin colorimetric detection, which is the official method prescribed by the European Pharmacopoeia. The described method is much easier to perform than the official method.
描述了使用沃特曼硅胶板作为固定相，通过薄层色谱法测定硫酸新霉素中B和C组分的相对含量。流动相由甲醇-20%（m/v）氯化钠溶液（15:85）组成。用4-氯-7-硝基苯并-2-恶唑-1,3-二唑衍生化后进行荧光检测。研究了不同参数对分离的影响。使用该方法分析了多个商业样品，并将结果与离子交换色谱法和茚三酮比色检测法（欧洲药典规定的官方方法）得到的结果进行了比较。所描述的方法比官方方法更容易操作。
