使用含荧光镓试剂的离子对反相高效液相色谱 - 荧光检测系统测定血清铝

Determination of serum aluminium using an ion-pair reversed-phase high-performance liquid chromatographic-fluorimetric system with lumogallion.

作者信息

Wu J, Zhou C Y, Chi H, Wong M K, Lee H K, Ong H Y, Ong C N

机构信息

Department of Chemistry, National University of Singapore, Kent Ridge.

出版信息

J Chromatogr B Biomed Appl. 1995 Jan 20;663(2):247-53. doi: 10.1016/0378-4347(94)00458-h.

DOI:10.1016/0378-4347(94)00458-h

PMID:7735472

Abstract

An ion-pair reversed-phase high-performance liquid chromatographic method with fluorimetric detection, using lumogallion [4-chloro-3-(2,4-dihydroxyphenylazo)-2-hydroxybenzene-1-sulphonic acid] as a ligand, has been successfully applied to the determination of aluminium in human serum. The highly fluorescent aluminium-lumogallion complex (lambda ex 505 nm, lambda em 574 nm) was separated on a LiChrosorb RP-18 column with an eluent consisting of 30% acetonitrile, 70% 0.02 M potassium hydrogen phthalate and 10 microM lumogallion. The proposed system offers a simple, rapid, selective and sensitive method for the determination of aluminium in serum. The detection limit for aluminium was 0.05 microgram/l in aqueous solution and the limit of determination was 2.2 micrograms/l in serum. The recovery of the method is generally over 90%.

摘要

采用荧光检测的离子对反相高效液相色谱法，以荧光镓试剂[4-氯-3-(2,4-二羟基苯偶氮)-2-羟基苯-1-磺酸]作为配体，已成功应用于测定人血清中的铝。在LiChrosorb RP-18柱上分离高荧光铝-荧光镓络合物（激发波长505 nm，发射波长574 nm），洗脱液由30%乙腈、70% 0.02 M邻苯二甲酸氢钾和10 μM荧光镓试剂组成。该方法为血清中铝的测定提供了一种简单、快速、选择性好且灵敏的方法。铝在水溶液中的检测限为0.05微克/升，在血清中的测定限为2.2微克/升。该方法的回收率一般超过90%。