Lacroix M, Tu T M, Donati F, Varin F
Faculté de Pharmacie, Université de Montréal, Qué., Canada.
J Chromatogr B Biomed Appl. 1995 Jan 20;663(2):297-307. doi: 10.1016/0378-4347(94)00443-9.
Two high-performance liquid chromatographic assays coupled with fluorometric detection have been developed for the determination of mivacurium isomers (trans-trans, cis-trans and cis-cis) and their monoester and alcohol metabolites in human plasma. A novel solid-phase extraction procedure allowed good recovery of the mivacurium isomers (mean 98%) and their monoester metabolites (mean 83%), whereas the alcohol metabolites were analyzed after direct precipitation of plasma proteins. For all analytes, these assays proved to be sensitive (LOQ 3.9-15.6 ng/ml), reproducible (C.V. < 15%) and accurate (> 94%) over the therapeutic range of concentrations of mivacurium and its metabolites. These two methods were applied successfully to a pharmacokinetic study of mivacurium after a bolus dose of 0.15 mg/kg in anesthetized patients.
已开发出两种高效液相色谱法结合荧光检测法，用于测定人血浆中米库氯铵异构体（反式-反式、顺式-反式和顺式-顺式）及其单酯和醇代谢物。一种新颖的固相萃取方法使米库氯铵异构体（平均98%）及其单酯代谢物（平均83%）的回收率良好，而醇代谢物则在血浆蛋白直接沉淀后进行分析。对于所有分析物，这些测定方法在米库氯铵及其代谢物的治疗浓度范围内均显示出灵敏性（定量限为3.9 - 15.6 ng/ml）、可重复性（变异系数< 15%）和准确性（> 94%）。这两种方法已成功应用于在麻醉患者中静脉注射0.15 mg/kg米库氯铵后的药代动力学研究。
