England L S, Lee H, Trevors J T
Department of Environmental Biology, University of Guelph, Ontario, Canada.
Mol Ecol. 1995 Apr;4(2):221-30. doi: 10.1111/j.1365-294x.1995.tb00211.x.
The effect of a genetically engineered Pseudomonas aureofaciens (Ps3732RNL11) strain (GEM) and the parental wild-type (Ps3732RN) on decomposition of cellulose paper, straw and calico cloth was assessed after 18 weeks incubation in laboratory soil microcosms. Effect(s) of inoculum density (10(3), 10(5), and 10(8) cells/g dry soil) and single versus multiple bacterial inoculations were also investigated. Cellulose paper was completely decomposed after 18 weeks in all treatments. There were no significant differences (95% level), between treatments, in percentage decomposition of either straw or calico cloth. Recovery of the GEM at 18 weeks, using viable plating, was limited to treatments originally receiving 10(8) cells/g dry soil. Log 1.8 CFU/g dry soil were recovered from the single dose treatment while log 4.2 CFU/g dry soil were recovered from the multiple dose treatment. Biolog metabolic tests were used to determine if the GEM or parental wild-type had any effect on overall carbon utilization in soil. Results suggested they did not. Detection of the recombinant lacZY gene sequence in soil using PCR suggested the possibility of viable but nonculturable cells and/or persistence of chromosomal DNA.
在实验室土壤微观世界中培养18周后，评估了基因工程改造的金黄色假单胞菌（Ps3732RNL11）菌株（GEM）和亲本野生型（Ps3732RN）对纤维素纸、稻草和印花布分解的影响。还研究了接种密度（10³、10⁵和10⁸个细胞/克干土）以及单次与多次细菌接种的效果。在所有处理中，纤维素纸在18周后完全分解。稻草或印花布的分解百分比在各处理之间没有显著差异（95%水平）。在18周时，使用活菌平板计数法回收GEM的情况仅限于最初接种量为10⁸个细胞/克干土的处理。单剂量处理回收的菌落形成单位为每克干土对数1.8 CFU，多剂量处理回收的菌落形成单位为每克干土对数4.2 CFU。使用Biolog代谢测试来确定GEM或亲本野生型是否对土壤中的总碳利用有任何影响。结果表明它们没有。利用聚合酶链反应（PCR）检测土壤中的重组lacZY基因序列表明存在存活但不可培养的细胞和/或染色体DNA持续存在的可能性。
