Armes J E, Eades S, Southey M C, Ross B C, Carapetis J R, Chow C W, Venter D J
Department of Anatomical Pathology, Austin Hospital, Heidelberg, Victoria, Australia.
Diagn Mol Pathol. 1995 Mar;4(1):39-47. doi: 10.1097/00019606-199503000-00008.
We report a case of a 21-year-old woman with hematopoietic, immunological, and congenital dysmorphic abnormalities, who died following rapidly progressive, disseminated Epstein-Barr virus (EBV)-associated lymphoproliferative disease (LPD). Polymerase chain reaction (PCR) amplification of formalin-fixed paraffin-embedded tissue showed differences in the clonality of each separate lymphoproliferative lesion examined, as determined by immunoglobulin heavy chain (IgH) gene rearrangement. PCR analysis also demonstrated that all lesions contained EBV genome. Since DNA had been extracted from paraffin blocks, a direct comparison of morphology and clonality could be made in each individual lesion. The evidence from this study indicates that the monoclonal tumors arose de novo in multiple sites and that the polyclonal background observed in some lesions reflected a substantial concomitant inflammatory response.
我们报告了一例21岁女性患者，其患有造血、免疫及先天性畸形异常，最终死于快速进展的播散性爱泼斯坦-巴尔病毒（EBV）相关淋巴增殖性疾病（LPD）。对福尔马林固定石蜡包埋组织进行聚合酶链反应（PCR）扩增，结果显示，通过免疫球蛋白重链（IgH）基因重排测定，所检查的每个单独淋巴增殖性病变的克隆性存在差异。PCR分析还表明，所有病变均含有EBV基因组。由于已从石蜡块中提取DNA，因此可以对每个个体病变的形态和克隆性进行直接比较。本研究的证据表明，单克隆肿瘤在多个部位新发产生，而在某些病变中观察到的多克隆背景反映了显著的伴随炎症反应。
