Choudhry M A, Ahmad S, Thompson K D, Sayeed M M
Department of Physiology, Loyola University of Chicago, Stritch School of Medicine, Maywood 60153, USA.
Shock. 1994 Apr;1(4):267-72. doi: 10.1097/00024382-199404000-00004.
Alterations in T-lymphocyte kinetics and/or activation have been implicated in burn and traumatic injury. This study evaluated concanavalin A (Con A) regulation of both intracellular Ca2+ (Cai2+) and proliferation in T-lymphocytes harvested from spleens of septic rats. Rats were implanted with fecal pellets containing Escherichia coli (150 colony forming units (CFU)) and Bacteroides fragilis (10(4) CFU). T-cell[Ca2+]i was measured before and after treatment of cells with ConA, using Fura-2 and microfluorophotometry. Splenic lymphocytes were cultured for 72 h with Con A to assess their proliferative response. As compared to sterile-implanted rats, the septic rat T-lymphocytes Cai2+ response to Con A significantly decreased on days 1 and 2 after implantation. A significant decrease in T-cell proliferative response to Con A, compared to sterile controls, was found in septic rats on day 2 but not on day 1. These results suggest that Con A-mediated proliferation in T-cells occurs secondarily to a decrease in cellular Ca2+ signalling. The depression in the T-cell proliferative response during sepsis could contribute to a decrease in host's resistance against sepsis.
T淋巴细胞动力学和/或激活的改变与烧伤和创伤性损伤有关。本研究评估了伴刀豆球蛋白A（Con A）对从脓毒症大鼠脾脏收获的T淋巴细胞内Ca2+（Cai2+）和增殖的调节作用。将含有大肠杆菌（150个菌落形成单位（CFU））和脆弱拟杆菌（104 CFU）的粪便颗粒植入大鼠体内。使用Fura-2和显微荧光光度法在ConA处理细胞前后测量T细胞[Ca2+]i。将脾淋巴细胞与Con A培养72小时以评估其增殖反应。与无菌植入大鼠相比，脓毒症大鼠T淋巴细胞对Con A的Cai2+反应在植入后第1天和第2天显著降低。与无菌对照组相比，脓毒症大鼠在第2天而非第1天发现T细胞对Con A的增殖反应显著降低。这些结果表明，Con A介导的T细胞增殖继发于细胞Ca2+信号传导的降低。脓毒症期间T细胞增殖反应的降低可能导致宿主对脓毒症抵抗力的下降。
