Vera O, Johannin G, Muñoz G, Roffi J
Laboratoire d'Endocrinologie, Centre Universitaire, Orsay.
C R Seances Soc Biol Fil. 1994;188(4):355-62.
The purpose of the present work was to determine if the neonatal testosterone surge in the rat (between 0 and 6 h after birth) was coincident with the histological and histochemical changes in the neonatal period. The histological study shows a decrease in the number of gonocytes and cells in mitosis. These observations are in good agreement with the results obtained by other authors. The critical period of birth does not reveal histological changes, between 0 and 6 h. By contrast, the histochemical analysis, based on the quantitative study of the delta 5-3 beta-hydroxysteroid deshydrogenase (key enzyme of the testosterone biosynthesis) demonstrated a regular enzymatic activity decreasing, from the foetal stage of 20.5 days to the birth, following a drastic increasing, from 0 to 2 h. The enzyme activity decreases gradually until 1 day after birth. The surge has been observed also if the foetus is removed by cesarean. These results demonstrated that the testicular crisis of the newborn Rat is simultaneous with an increased synthesis of testosterone in the testis.
本研究的目的是确定新生大鼠出生后0至6小时内的睾酮激增是否与新生儿期的组织学和组织化学变化相一致。组织学研究显示生殖母细胞和有丝分裂细胞的数量减少。这些观察结果与其他作者获得的结果高度一致。出生的关键时期（0至6小时）未显示出组织学变化。相比之下，基于对δ5-3β-羟基类固醇脱氢酶（睾酮生物合成的关键酶）的定量研究的组织化学分析表明，从20.5天的胎儿期到出生，酶活性呈规律下降，而在出生后0至2小时则急剧上升。酶活性逐渐下降直至出生后1天。即使通过剖宫产取出胎儿，也观察到了这种激增现象。这些结果表明，新生大鼠的睾丸危象与睾丸中睾酮合成增加同时发生。
