从显微切割产生的微克隆中鉴定出位于人类11号染色体11q13.4至q25区域的50个新的序列标签位点（STS）。

Fifty novel sequence-tagged sites (STSs) on human chromosome 11q13.4-->q25 identified from microclones generated by microdissection.

作者信息

Soejima H, Yoshiura K, Tamura T, Tokino T, Nakamura Y, Niikawa N, Jinno Y

机构信息

Department of Human Genetics, Nagasaki University School of Medicine, Japan.

出版信息

Cytogenet Cell Genet. 1995;70(1-2):108-11. doi: 10.1159/000134003.

DOI:10.1159/000134003

PMID:7736773

Abstract

Fifty sequence-tagged sites (STSs) from human chromosome region, 11q13.4-->q25 were identified in microdissection-generated microclones. From a total of 124 single-copy microclones obtained from regions 11q14-->q22 and 11q23-->q25, 59 were sequenced, and a PCR primer pair was designed for each of them. Fifty of the 59 clones were mapped to regions ranging from 11q13.4 to 11q25 by means of PCR on a somatic hybrid cell panel with various deletions of the long arm of human chromosome 11. These STSs will contribute to the construction of physical cosmid/YAC maps representing the chromosome regions. They are also useful for analysis of chromosome aberrations, such as translocations, inversions or marker chromosomes.

摘要

在显微切割产生的微克隆中鉴定出了来自人类染色体区域11q13.4→q25的50个序列标签位点（STS）。从11q14→q22和11q23→q25区域获得的总共124个单拷贝微克隆中，对59个进行了测序，并为每个微克隆设计了一对PCR引物。通过在具有人类11号染色体长臂各种缺失的体细胞杂种细胞板上进行PCR，将59个克隆中的50个定位到了从11q13.4到11q25的区域。这些STS将有助于构建代表染色体区域的物理粘粒/YAC图谱。它们也有助于分析染色体畸变，如易位、倒位或标记染色体。