Sugano K, Fukayama N, Ohkura H, Shimosato Y, Yamada Y, Inoue T, Sekiya T, Hayashi K
Division of Clinical Laboratory, National Cancer Center Hospital.
Electrophoresis. 1995 Jan;16(1):8-10. doi: 10.1002/elps.1150160103.
Using a newly developed temperature-gradient gel electrophoresis apparatus, mutations in the K-ras oncogene and p53 tumor suppressor gene were analyzed for single-strand conformation polymorphism (SSCP). The mobilities of single-stranded DNAs, carrying various mutations, change--depending on the gel temperature during electrophoresis. Therefore, temperature-gradient single-strand conformation polymorphism (TG-SSCP) analysis can provide useful information concerning the optimum temperature for SSCP and may also be used to screen for various mutations or polymorphisms in a single electrophoretic run.
使用新开发的温度梯度凝胶电泳装置，对K-ras癌基因和p53肿瘤抑制基因中的突变进行单链构象多态性（SSCP）分析。携带各种突变的单链DNA的迁移率会根据电泳过程中的凝胶温度而变化。因此，温度梯度单链构象多态性（TG-SSCP）分析可以提供有关SSCP最佳温度的有用信息，还可用于在单次电泳运行中筛选各种突变或多态性。
