一种用于构建lacZ转录融合体的新型载体-宿主系统，适用于仅需要低水平基因表达的情况。
A new vector-host system for construction of lacZ transcriptional fusions where only low-level gene expression is desirable.
作者信息
Podkovyrov S M, Larson T J
机构信息
Department of Biochemistry and Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg 24061-0308, USA.
出版信息
Gene. 1995 Apr 14;156(1):151-2. doi: 10.1016/0378-1119(95)00053-9.
We improved a multicopy vector, pRS415 [Simons et al., Gene 53 (1987) 85-96], for use in operon fusion constructions by introducing a new multiple cloning site (MCS) containing eight unique restriction sites upstream from the promoterless reporter gene lacZ. In order to reduce plasmid copy number, a new Escherichia coli strain SP2 (pcnB, delta lac, recA) was constructed. This strain permits analysis of fusions in cases where high gene dosage may be detrimental.
我们改进了一种多拷贝载体pRS415[西蒙斯等人，《基因》53(1987)85 - 96]，通过在无启动子报告基因lacZ上游引入一个包含八个独特限制性酶切位点的新多克隆位点（MCS），用于操纵子融合构建。为了降低质粒拷贝数，构建了一种新的大肠杆菌菌株SP2（pcnB，Δlac，recA）。该菌株允许在高基因剂量可能有害的情况下分析融合情况。
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