Spira G, Yuan R, Paizi M, Weisendal M, Casadevall A
Rappaport Family Institute for Research in the Medical Sciences, Bruce Rappaport Faculty of Medicine, Technion, Haifa, Israel.
Hybridoma. 1994 Dec;13(6):531-5. doi: 10.1089/hyb.1994.13.531.
Allelic exclusion normally results in the expression of only one light and one heavy chain gene. However, some hybridomas have been reported to express two different heavy chain genes. Here we report that the IgG3 hybridoma 4H3.C8B expresses both kappa and lambda light chains. 4H3.C8B was originally recovered by screening for antibody binding to Cryptococcus neoformans polysaccharide antigen and characterized as gamma 3 lambda. The gamma 3 lambda but not the gamma 3 kappa binds to polysaccharide antigen. Some of the antibody molecules were heterodimers composed of both lambda and kappa. IgG1 and IgG2b isotype switch variants were identified and isolated by the technique of sib selection, using the ELISA spot assay. Like the parent IgG3 line, the switch variants continued to express both kappa and lambda light chains but only the lambda-containing antibodies bound to the antigen. Our experience suggests that hybridomas recovered by assays that are antigen dependent should also be tested for the expression of other isotypes and light chains in a non-antigen-binding immunoassay.
等位基因排斥通常导致仅表达一个轻链基因和一个重链基因。然而，据报道一些杂交瘤会表达两种不同的重链基因。在此我们报告，IgG3杂交瘤4H3.C8B同时表达κ链和λ链。4H3.C8B最初是通过筛选与新型隐球菌多糖抗原结合的抗体而获得的，并被鉴定为γ3λ。γ3λ而非γ3κ与多糖抗原结合。一些抗体分子是由λ链和κ链组成的异源二聚体。使用ELISA斑点试验，通过同胞选择技术鉴定并分离出IgG1和IgG2b同种型转换变体。与亲本IgG3细胞系一样，转换变体继续表达κ链和λ链，但只有含λ链的抗体与抗原结合。我们的经验表明，通过抗原依赖性检测回收的杂交瘤也应在非抗原结合免疫测定中检测其他同种型和轻链的表达。
