Calandra G B, Whitt R S, Cole R M
Infect Immun. 1976 Mar;13(3):813-7. doi: 10.1128/iai.13.3.813-817.1976.
The relationship of streptolysin S (SLS) and a cellular potential hemolysin (PH) activatable by sonication was examined in strain C203S (a known high producer of SLS), its SLS(-) mutant (C203U), and in 20 other group A streptococci of various M and T serotypes. All strains shown to form SLS (ribobycleic acid (RNA)-core SLS) contained PH. The two strains lacking PH were the only ones that did not produce SLS In strain C203S, formation of SLS bycells incubated with RNA-core for 60 min at 47 C in a nongrowth basal medium (Bernheimer's basal medium) was followed by a marked decrease (99.6% loss) of PH titer. Without stimulation of SLS production by addition of RNA-sore, the same incubation resulted in a progressive but less marked fall (38.8%loss in 60 min) of PH titer: these cells produced disproportionately low titers of SLS on subsequent addition of RNA-core. This effect of prior incubation in Bernheimer's basal medium on SLS titer was partially nullified by use of fresh medium after 30 min, but not after 60 min, and did not occur during 60 min of incubation at OC. These results provide additional evidence for a precursor-product relationship between PH and SLS. They also suggest that a medium factor (or factors) is utilized or destroyed at 37 C and that this factor is essential to both the stability of PH and its efficient conversion to SLS.
在C203S菌株（一种已知的高产量链球菌溶血素S（SLS）产生菌）、其SLS（-）突变体（C203U）以及其他20种不同M和T血清型的A组链球菌中，研究了SLS与可通过超声处理激活的细胞潜在溶血素（PH）之间的关系。所有显示形成SLS（核糖核酸（RNA）核心SLS）的菌株都含有PH。仅有的两种缺乏PH的菌株是不产生SLS的菌株。在C203S菌株中，细胞在非生长基础培养基（伯恩海默基础培养基）中于47℃与RNA核心孵育60分钟形成SLS后，PH滴度显著下降（损失99.6%）。在不添加RNA核心刺激SLS产生的情况下，相同的孵育导致PH滴度逐渐但不太明显的下降（60分钟内损失38.8%）：这些细胞在随后添加RNA核心时产生的SLS滴度极低。在伯恩海默基础培养基中预先孵育对SLS滴度的这种影响在30分钟后使用新鲜培养基可部分消除，但60分钟后则不能，并且在0℃孵育60分钟期间不会发生。这些结果为PH和SLS之间的前体-产物关系提供了额外证据。它们还表明，一种培养基因子在37℃被利用或被破坏，并且该因子对于PH的稳定性及其有效转化为SLS都是必不可少的。
