Nadal A, Campo E, Pinto J, Mallofré C, Palacín A, Arias C, Traserra J, Cardesa A
Department of Anatomic Pathology, Hospital Clinic i Provincial, University of Barcelona, Medical School, Spain.
J Pathol. 1995 Feb;175(2):181-8. doi: 10.1002/path.1711750205.
p53 expression has been examined in 89 squamous cell carcinomas of the larynx (34 glottic, 28 supraglottic, 18 transglottic, 8 pyriform sinus, and 1 subglottic) obtained from 88 patients surgically treated in our centre. In addition, 59 laryngeal samples including normal respiratory epithelium and non-invasive squamous cell lesions were also tested. Frozen sections were immunostained with PAb 1801 and the results were correlated with pathological features, DNA ploidy and S-phase of the tumours, disease-free interval, and survival of the patients. p53 immunoreactivity was observed in 57 (64 per cent) carcinomas. None of the eight samples of normal respiratory epithelium was positive. p53-positive cells were seen in 8 of 23 (35 per cent) squamous cell metaplasias, 6 of 19 (32 per cent) low-grade dysplasias and 5 of 10 (50 per cent) high-grade dysplasias. No correlation was found between p53 expression in carcinomas and their clinical and pathological characteristics, DNA ploidy, or proliferative activity. Neither disease-free nor overall survival showed differences between p53-positive and p53-negative cases. These findings indicate that p53 may play a role in an early stage of malignant transformation of a subset of squamous cell carcinomas of the larynx, but seems not to be associated with further progression of the tumours.
我们对在本中心接受手术治疗的88例患者的89例喉鳞状细胞癌（34例声门型、28例声门上型、18例跨声门型、8例梨状窝型和1例声门下型）进行了p53表达检测。此外，还检测了包括正常呼吸道上皮和非侵袭性鳞状细胞病变在内的59份喉部样本。冰冻切片用PAb 1801进行免疫染色，并将结果与肿瘤的病理特征、DNA倍体和S期、无病生存期以及患者生存率进行关联分析。在57例（64%）癌组织中观察到p53免疫反应性。8份正常呼吸道上皮样本均为阴性。在23例（35%）鳞状细胞化生中的8例、19例（32%）低级别发育异常中的6例和10例（50%）高级别发育异常中的5例中可见p53阳性细胞。未发现癌组织中p53表达与其临床和病理特征、DNA倍体或增殖活性之间存在相关性。p53阳性和p53阴性病例的无病生存期和总生存期均无差异。这些发现表明，p53可能在一部分喉鳞状细胞癌的恶性转化早期起作用，但似乎与肿瘤的进一步进展无关。
