Stratton M R, Collins N, Lakhani S R, Sloane J P
Section of Molecular Carcinogenesis, Royal Marsden Hospital, Sutton, Surrey, U.K.
J Pathol. 1995 Feb;175(2):195-201. doi: 10.1002/path.1711750207.
Loss of heterozygosity (LOH) at loci reported to show allele loss in invasive breast cancers was examined in ductal in situ carcinomas of the breast using polymorphic short tandem repeats and the polymerase chain reaction (PCR). LOH was detected at all loci examined in at least 11 per cent of the samples examined. The proportion of cases of in situ carcinoma showing LOH at these loci was similar to that previously reported in invasive cancers. Cases of pure in situ cancer without an invasive component exhibited an overall lower frequency of allele loss. LOH at more than one locus was observed in some intraductal cancers. In a small number of cases, LOH was present in the invasive but not in the intraductal component of the tumour, suggesting that mutation at the locus concerned was associated with development of invasive behaviour.
利用多态性短串联重复序列和聚合酶链反应（PCR），在乳腺导管原位癌中检测了据报道在浸润性乳腺癌中显示等位基因缺失的基因座的杂合性缺失（LOH）。在所检测的所有基因座中，至少11%的检测样本中检测到了LOH。原位癌病例在这些基因座显示LOH的比例与先前报道的浸润性癌相似。没有浸润成分的纯原位癌病例显示等位基因缺失的总体频率较低。在一些导管内癌中观察到一个以上基因座的LOH。在少数病例中，肿瘤的浸润成分中存在LOH，而导管内成分中不存在，这表明相关基因座的突变与浸润性行为的发展有关。
