Rossi G R, Sastre D A, Rubinstein H R, Masih D T
Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina.
J Med Vet Mycol. 1994 Dec;32(6):405-14.
The biochemical basis of peritoneal cell cytotoxicity for Cryptococcus neoformans was studied by measuring the killing of the yeast by peritoneal resident cells and peritoneal exudate cells obtained from normal and proteose-peptone-injected animals, respectively. Both cell populations killed C. neoformans to an equivalent extent after 3 h incubation. Exudate cells showed anti-cryptococcal activity from the first hour of incubation, while no killing was observed with resident cells before 3 h. Both cell populations triggered a respiratory burst in response to opsonized C. neoformans as indicated by the fact that killing of the yeast was inhibited by scavengers of reactive oxygen intermediates (ROI). C. neoformans susceptibility to H2O2 and hydroxyl radicals in cell-free systems is demonstrated by incubating a yeast suspension with different concentrations of H2O2 and Fenton's reagents, respectively. These results suggest that oxygen metabolites play an active role in C. neoformans killing.
通过分别检测正常动物和经蛋白胨注射的动物的腹腔驻留细胞及腹腔渗出细胞对新型隐球菌的杀伤作用，研究了腹腔细胞对新型隐球菌细胞毒性的生化基础。孵育3小时后，这两种细胞群体对新型隐球菌的杀伤程度相当。渗出细胞在孵育的第一个小时就显示出抗隐球菌活性，而驻留细胞在3小时之前未观察到杀伤作用。两种细胞群体在面对调理过的新型隐球菌时都会引发呼吸爆发，这一事实表明，活性氧中间体（ROI）清除剂可抑制酵母的杀伤。通过分别将酵母悬浮液与不同浓度的过氧化氢和芬顿试剂孵育，证明了新型隐球菌在无细胞体系中对过氧化氢和羟基自由基敏感。这些结果表明，氧代谢产物在新型隐球菌的杀伤中起积极作用。
