从大鼠肝脏分离的内质网囊泡中一种ATP依赖的铜转运系统的鉴定。
Identification of an ATP-dependent copper transport system in endoplasmic reticulum vesicles isolated from rat liver.
作者信息
Bingham M J, Burchell A, McArdle H J
机构信息
Department of Child Health, University of Dundee, UK.
出版信息
J Physiol. 1995 Feb 1;482 ( Pt 3)(Pt 3):583-7. doi: 10.1113/jphysiol.1995.sp020542.
Abstract
- This paper identifies and characterizes an ATP-dependent copper transport system in endoplasmic reticulum vesicles isolated from male rat liver. 2. The transporter has a Km of 2.5 +/- 1.2 mumol 1(-1) copper glutathione (CuGSH) and a Vmax of 4.5 +/- 1.3 nmol (mg protein)-1 (5 min)-1 for copper. 3. At a copper concentration of 2 mumol l-1, ATP dependence reaches saturation, with a Km for ATP of 4.7 +/- 2.4 mmol l-1 and a Vmax of 2.8 +/- 0.6 nmol (mg protein)-1 (5 min)-1. 4. The uptake is dependent on ATP hydrolysis, since a low energy analogue of ATP, adenosine 5'-[beta-gamma-methylene] triphosphate tetralithium (AMP.PCP), has no effect on copper uptake. 5. The transporter is a P-type ATPase, since vanadate inhibits uptake with a high degree of specificity (100 mumol l-1 inhibits uptake by 50% at a copper concentration of 2 mumol l-1).
摘要
- 本文鉴定并描述了从雄性大鼠肝脏分离的内质网囊泡中的一种ATP依赖性铜转运系统。2. 该转运体对铜谷胱甘肽（CuGSH）的Km为2.5±1.2 μmol 1⁻¹，对铜的Vmax为4.5±1.3 nmol（mg蛋白质）⁻¹（5分钟）⁻¹。3. 在铜浓度为2 μmol l⁻¹时，ATP依赖性达到饱和，ATP的Km为4.7±2.4 mmol l⁻¹，Vmax为2.8±0.6 nmol（mg蛋白质）⁻¹（5分钟）⁻¹。4. 摄取依赖于ATP水解，因为ATP的低能量类似物腺苷5'-[β-γ-亚甲基]三磷酸四锂（AMP.PCP）对铜摄取没有影响。5. 该转运体是一种P型ATP酶，因为钒酸盐以高度特异性抑制摄取（在铜浓度为2 μmol l⁻¹时，100 μmol l⁻¹抑制摄取50%）。
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