Douchet C, Thérizol-Ferly M, Kombila M, Duong T H, Gomez de Diaz M, Barrabes A, Richard-Lenoble D
Service de Parasitologie-Mycologie et Médecine Tropicale, Faculté de Médecine de Tours, France.
Mycoses. 1994 Jul-Aug;37(7-8):261-4. doi: 10.1111/j.1439-0507.1994.tb00423.x.
Fifty-two Trichosporon strains isolated from Gabonese female patients 15-60 years, were studied. The identity of these strains was established by two different methods: the method proposed by Guého et al. (1992), based on mycological criteria, and a slide agglutination method performed with monospecific antisera prepared in our laboratory. The final results show a perfect correlation between the two methods, which allowed us to identify 25 strains of T. mucoides, 21 strains of T. inkin and seven strains of T. asahii. The results of the agglutination tests performed with 24-h-old subcultures grown on Sabouraud glucose agar are available in less than 15 mins. In the light of these results, it appears that this method, which is rapid and easy to perform and reproduce, may readily be used in hospital laboratories. In addition, this method allowed us to verify the presence of antigens common to the genera Cryptococcus and Trichosporon, which were easily shown by the use of crude sera. The fact that the anti-Trichosporon monospecific sera lose their capacity to agglutinate Cryptococcus neoformans proves their high specificity.
对从15至60岁加蓬女性患者中分离出的52株毛孢子菌菌株进行了研究。这些菌株的鉴定采用了两种不同方法：一种是Guého等人（1992年）提出的基于真菌学标准的方法，另一种是用我们实验室制备的单特异性抗血清进行的玻片凝集法。最终结果表明这两种方法之间具有完美的相关性，据此我们鉴定出25株黏液毛孢子菌、21株因肯毛孢子菌和7株浅黄毛发毛孢子菌。用在沙氏葡萄糖琼脂上培养24小时的传代培养物进行凝集试验，结果在不到15分钟内即可获得。鉴于这些结果，看来这种方法快速、易于操作和重复，可很容易地在医院实验室中使用。此外，这种方法使我们能够验证隐球菌属和毛孢子菌属共有的抗原的存在，使用粗血清很容易显示这些抗原。抗毛孢子菌单特异性血清失去凝集新型隐球菌的能力，这一事实证明了它们的高度特异性。
