Muchardt C, Sardet C, Bourachot B, Onufryk C, Yaniv M
UA1644 du CNRS, Département des Biotechnologies, Institut Pasteur, Paris, France.
Nucleic Acids Res. 1995 Apr 11;23(7):1127-32. doi: 10.1093/nar/23.7.1127.
In yeast, the SNF/SWI complex is involved in transcriptional activation of several inducible promoters, possibly by causing a local modification of the chromatin structure. Recently, two human homologues of the SNF2/SWI2 protein have been isolated, hbrm and BRG-1. In addition, a complex containing one of the SNF2/SWI2 homologues and having an in vitro activity similar to the yeast complex has been partially purified from HeLa cells. Here we describe the characterization of a cDNA encoding a human nuclear protein containing a large domain of homology with SNF5, another member of the yeast SNF/SWI complex. This protein can be co-immunoprecipitated with hbrm and the interaction between the two proteins is dependent on the region conserved between the human and the yeast SNF5. These findings suggest that the cDNA we have cloned encodes one of the members of the human SNF/SWI complex.
在酵母中，SNF/SWI复合物参与了几个可诱导启动子的转录激活，可能是通过引起染色质结构的局部改变来实现的。最近，已分离出SNF2/SWI2蛋白的两个人类同源物，即hbrm和BRG-1。此外，还从HeLa细胞中部分纯化出一种含有SNF2/SWI2同源物之一且具有类似于酵母复合物体外活性的复合物。在此，我们描述了一个编码人类核蛋白的cDNA的特性，该蛋白含有一个与酵母SNF/SWI复合物的另一个成员SNF5具有高度同源性的大结构域。这种蛋白可以与hbrm进行共免疫沉淀，并且这两种蛋白之间的相互作用取决于人和酵母SNF5之间保守的区域。这些发现表明，我们克隆的cDNA编码人类SNF/SWI复合物的成员之一。
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