异丙肾上腺素增强了三磷酸腺苷（ATP）诱发的氯离子跨犬气管上皮的分泌。

Isoproterenol augments ATP-evoked Cl- secretion across canine tracheal epithelium.

作者信息

Satoh M, Yamaya M, Shimura S, Masuda T, Yamamoto M, Sasaki T, Sasaki H, Shirato K

机构信息

First Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.

出版信息

Respir Physiol. 1995 Jan;99(1):13-8. doi: 10.1016/0034-5687(94)00079-f.

DOI:10.1016/0034-5687(94)00079-f

PMID:7740201

Abstract

Using an Ussing chamber, both the posterior epithelial membrane and cultured epithelial cell monolayers from canine trachea were used for measurements of potential difference (PD) and short circuit current (SCC). Conductance (G) was calculated as the ratio SCC/PD. Adenosine (10(-5) to 10(-3) M) failed to produce any significant increases in PD and SCC. ATP (10(-6) to 10(-4) M) produced a significant transient increase in SCC in a dose-dependent fashion, reaching a peak value within 3 min after stimulation. Isoproterenol (ISOP) at 10(-8) M itself did not significantly alter the SCC value. In both the epithelial membrane and cultured epithelial cells treated with amiloride, however, pretreatment with 10(-8) M ISOP significantly augmented the ATP-induced SCC rise, whereas G did not significantly change. These findings indicate that beta-adrenergic stimulation augments Cl- secretion induced by P2-receptor stimulation in airway epithelial cells.

摘要

使用尤斯灌流室，对犬气管的后上皮膜和培养的上皮细胞单层进行了电位差（PD）和短路电流（SCC）测量。电导（G）计算为SCC/PD的比值。腺苷（10⁻⁵至10⁻³ M）未能使PD和SCC产生任何显著增加。ATP（10⁻⁶至10⁻⁴ M）以剂量依赖方式使SCC产生显著的瞬时增加，刺激后3分钟内达到峰值。10⁻⁸ M的异丙肾上腺素（ISOP）本身并未显著改变SCC值。然而，在阿米洛利处理的上皮膜和培养的上皮细胞中，用10⁻⁸ M ISOP预处理显著增强了ATP诱导的SCC升高，而G没有显著变化。这些发现表明，β-肾上腺素能刺激增强了气道上皮细胞中P2受体刺激诱导的Cl⁻分泌。