Kucharski J, Nowak G
Department of Agricultural Microbiology, Olsztyn University of Agriculture and Technology, Lódzki, Poland.
Acta Microbiol Pol. 1994;43(3-4):381-8.
The pot trial was performed to study the effect of L-tryptophane (as an auxin precursor) applied in the amount 0.3 and 3.0 mg per 1 kg of the soil on yield and the chemical composition of field bean. The effects of this compound on dehydrogenases activity in the cells Rhizobium leguminosarum isolated from root nodules, soil dehydrogenases activity and number of microorganisms from different systematic or physiological groups were also studied. The effects of L-tryptophane were compared to indole-3-acetic acid (IAA) after application to soil in the rate 0.2 mg per 1 kg of the soil of foliar spraying in the rate 20 mg Din 1 dm3 of distilled water. Studies were carried out in three experimental series: without microorganisms or with addition of Azotobacter sp. or Rhizobium leguminosarum biovar. viciae to the soil. It was found that L-tryptophane and IAA did not affect the yield of above ground part and roots of field bean and their effects on macronutrients concentration were not direct and dependent on the nutrient and experimental series. L-tryptophane and auxine increased the dehydrogenases activity in the cells of Rhizobium leguminosarum isolated from root nodules and the effect on the activity of soil dehydrogenases and urease was dependent on the rate of L-tryptophane. This chemical adversely affected the numbers of some microorganisms groups.
进行盆栽试验以研究每1千克土壤施用0.3毫克和3.0毫克的L-色氨酸（作为生长素前体）对蚕豆产量和化学成分的影响。还研究了该化合物对从根瘤中分离出的豆科根瘤菌细胞中的脱氢酶活性、土壤脱氢酶活性以及来自不同系统或生理类群的微生物数量的影响。将L-色氨酸的效果与吲哚-3-乙酸（IAA）进行比较，IAA以每1千克土壤0.2毫克的施用量施用于土壤，或以20毫克/1立方分米蒸馏水的喷施量进行叶面喷施。研究分三个实验系列进行：不添加微生物，或向土壤中添加固氮菌属或豌豆根瘤菌生物型蚕豆。结果发现，L-色氨酸和IAA对蚕豆地上部分和根部的产量没有影响，它们对大量营养素浓度的影响并非直接的，且取决于养分和实验系列。L-色氨酸和生长素增加了从根瘤中分离出的豆科根瘤菌细胞中的脱氢酶活性，对土壤脱氢酶和脲酶活性的影响取决于L-色氨酸的用量。这种化学物质对一些微生物类群的数量产生了不利影响。
