Kawano S, Tatsumi E, Yoneda N, Tani A, Nakamura F
Department of Laboratory Medicine, Kobe University School of Medicine, Japan.
Am J Hematol. 1995 May;49(1):6-14. doi: 10.1002/ajh.2830490103.
The expression of CD45 RA/RO antigen was investigated in neoplasms including cases expressing CD7 antigen as the sole pan-T antigen (n = 8), T-lineage acute lymphoblastic leukemia (ALL)/lymphoblastic lymphoma (LBL) at various stages of differentiation (n = 32), peripheral stage T-lineage leukemia (n = 10) and adult T-cell leukemia (ATL) (n = 14). The p56lck gene expression was also investigated in selected cases. The expression pattern of CD45 RA/RO antigen was defined as of RA, mixed, or RO type. All but one CD7+ CD5- CD2- case were of the RA type. The CD7+ CD5+ CD2- prothymic stage included seven RA and one mixed type cases. One CD7+ CD5- CD2+ case was of the RA type, but the other was of the RO type. The CD7+ CD5+ CD2+ prothymic stage included three RA and four mixed type cases. All seven CD3- CD4+ CD8+ (double-positive) thymic cases were of the RO type. The CD3+ CD4+ CD8+ (triple-positive) stage included two RO and three mixed-type cases. One CD3+ CD4+ CD8- late thymic case was of the mixed type. The peripheral stage cases included five RA, three RO, and two mixed type cases. All ATL cases were of the RO type. The expression of p56lck gene in the prothymic stage was less marked than that in the thymic stage. On the basis of these results, the following sequence of pattern of the CD45 RA/RO antigen expression along with T-lineage differentiation was reconstructed: prothymic stage [RA and mixed type]-->double-positive thymic stage [RO type]-->triple-positive thymic stage [RO and mixed type]-->peripheral stage [RA, mixed, and RO type]. While one RO-type CD7+ CD5- CD2- and one RO-type CD7+ CD5- CD2+ cases were not in accord with this sequence, the pattern of CD45 RA/RO antigen expression in most of T-lineage neoplasms could be determined by the respective stage of differentiation. The poor expression of the p56lck gene by the prothymic blasts compared with the thymic blasts may be related to the expression pattern of the CD45 RA/RO molecules, which exhibits phosphatase activity. The consistent RO-type expression in the ATL cases may reflect the activated status of the neoplastic T cells due to the presence of the HTLV-I gene. Alternatively, the target cells for HTLV-I-induced neoplastic transformation may possible be of the RO type.
在多种肿瘤中研究了CD45 RA/RO抗原的表达，包括仅表达CD7抗原作为唯一泛T抗原的病例（n = 8）、处于不同分化阶段的T系急性淋巴细胞白血病（ALL）/淋巴细胞淋巴瘤（LBL）（n = 32）、外周血阶段T系白血病（n = 10）和成人T细胞白血病（ATL）（n = 14）。还在部分病例中研究了p56lck基因的表达。CD45 RA/RO抗原的表达模式分为RA型、混合型或RO型。除1例CD7 + CD5 - CD2 - 病例外，其余均为RA型。CD7 + CD5 + CD2 - 原胸腺阶段包括7例RA型和1例混合型病例。1例CD7 + CD5 - CD2 + 病例为RA型，另1例为RO型。CD7 + CD5 + CD2 + 原胸腺阶段包括3例RA型和4例混合型病例。所有7例CD3 - CD4 + CD8 + （双阳性）胸腺病例均为RO型。CD3 + CD4 + CD8 + （三阳性）阶段包括2例RO型和3例混合型病例。1例CD3 + CD4 + CD8 - 晚期胸腺病例为混合型。外周血阶段病例包括5例RA型、3例RO型和2例混合型病例。所有ATL病例均为RO型。原胸腺阶段p56lck基因的表达不如胸腺阶段明显。基于这些结果，重建了CD45 RA/RO抗原表达模式随T系分化的以下顺序：原胸腺阶段[RA型和混合型]→双阳性胸腺阶段[RO型]→三阳性胸腺阶段[RO型和混合型]→外周血阶段[RA型、混合型和RO型]。虽然1例RO型CD7 + CD5 - CD2 - 和1例RO型CD7 + CD5 - CD2 + 病例不符合此顺序，但大多数T系肿瘤中CD45 RA/RO抗原的表达模式可由各自的分化阶段决定。与胸腺母细胞相比，原胸腺母细胞中p56lck基因表达较差可能与具有磷酸酶活性的CD45 RA/RO分子的表达模式有关。ATL病例中一致的RO型表达可能反映了由于HTLV - I基因的存在导致肿瘤性T细胞的激活状态。或者，HTLV - I诱导肿瘤转化的靶细胞可能为RO型。
