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Metabotropic glutamate receptor analogues inhibit p[NH]ppG-stimulated phospholipase C activity in bovine brain coated vesicles: involvement of a pertussis toxin-sensitive G-protein.

作者信息

Martín M, Sanz J M, Ros M, Cubero A

机构信息

Departamento de Inorgánica Orgánica y Bioquímica, Facultad de Químicas, Universidad de Castilla-La Mancha, Ciudad Real, Spain.

出版信息

Biochem J. 1995 May 1;307 ( Pt 3)(Pt 3):851-7. doi: 10.1042/bj3070851.

DOI:10.1042/bj3070851
PMID:7741717
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1136726/
Abstract

Guanosine 5'-[beta gamma-imido]triphosphate (p[NH]ppG)-stimulated phospholipase C (PLC) activity in bovine brain coated vesicles is inhibited by glutamate agonists. In the present study we show that quisqualic acid (QA), (+/-)-trans-1-aminocyclopentane-1,3-dicarboxylate (trans-ACPD), glutamic acid and ibotenic acid inhibited p[NH]ppG-stimulated PLC by 44, 41, 36 and 25% respectively. Carbachol also produced an inhibition of p[NH]ppG-stimulated PLC by 45%. The inhibition caused by trans-ACPD and QA was dose-dependent. DL-2-Amino-3-phosphonopropionic acid and (RS)-alpha-methyl-4-carboxyphenylglycine, specific antagonists of metabotropic glutamate receptors (mGluRs), abolished these inhibitory effects. trans-ACPD inhibition of p[NH]ppG-stimulated PLC was also observed in the presence of ionotropic glutamate receptor antagonists. When carbachol and QA or trans-ACPD were combined, additive inhibitory effects were observed. Preincubation of bovine brain coated vesicles with pertussis toxin abolished the inhibitory effects of mGluR analogues and carbachol on p[NH]ppG-stimulated PLC activity. The presence of Gs alpha and pertussis toxin substrates, Gi alpha and Go alpha subunits as well as PLC beta 1 in bovine brain coated vesicles has been confirmed by immunoblot. These results support the coupling of mGluRs to a PLC in an inhibitory manner through a pertussis toxin-sensitive G-protein in bovine brain coated vesicles.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccfa/1136726/68819cd877ae/biochemj00064-0235-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccfa/1136726/e13619aa4020/biochemj00064-0234-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccfa/1136726/87bde1565f61/biochemj00064-0235-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccfa/1136726/68819cd877ae/biochemj00064-0235-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccfa/1136726/e13619aa4020/biochemj00064-0234-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccfa/1136726/87bde1565f61/biochemj00064-0235-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccfa/1136726/68819cd877ae/biochemj00064-0235-b.jpg

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