Mukundan N E, Flanders P C, Constantinidis I, Papas K K, Sambanis A
Frederik Philips Magnetic Resonance Research Center, Department of Radiology, Emory University School of Medicine, Emory University, Atlanta, GA 30322, USA.
Biochem Biophys Res Commun. 1995 May 5;210(1):113-8. doi: 10.1006/bbrc.1995.1634.
Oxygen consumption rates of beta TC3 mouse insulinoma cells were measured in three different cell culture systems: monolayer, freshly trypsinized cell suspension, and trypsinized cells entrapped in alginate-poly-L-lysine-alginate (APA) polymer beads. The oxygen consumption rate for cells in the APA beads was similar to the rate for freshly trypsinized cells, indicating that cells can be entrapped without affecting their oxygen consumption rate. The cells in monolayers consumed oxygen at a higher rate than freshly trypsinized cells or cells in APA beads, suggesting that trypsinization may lower oxygen consumption capability. In addition, the oxygen consumption of beta TC3s during glucose-induced insulin secretion was not significantly different from the basal rate, in contrast to the increased oxygen demand of actively secreting islets. Our results suggest that beta TC3s may be better suited than islets for use in a bioartificial pancreas due to their stable oxygen requirements.
在三种不同的细胞培养系统中测量了βTC3小鼠胰岛素瘤细胞的耗氧率：单层培养、新鲜胰蛋白酶消化后的细胞悬液以及包裹在藻酸盐-聚-L-赖氨酸-藻酸盐（APA）聚合物珠中的胰蛋白酶消化细胞。APA珠中细胞的耗氧率与新鲜胰蛋白酶消化细胞的耗氧率相似，这表明细胞被包裹后其耗氧率不受影响。单层培养的细胞比新鲜胰蛋白酶消化的细胞或APA珠中的细胞消耗氧气的速率更高，这表明胰蛋白酶消化可能会降低细胞的耗氧能力。此外，与活跃分泌的胰岛对氧气需求增加相反，βTC3细胞在葡萄糖诱导的胰岛素分泌过程中的耗氧量与基础速率没有显著差异。我们的结果表明，由于βTC3细胞对氧气需求稳定，它们可能比胰岛更适合用于生物人工胰腺。
