Nagaki M, Shidoji Y, Yamada Y, Sugiyama A, Tanaka M, Akaike T, Ohnishi H, Moriwaki H, Muto Y
First Department of Internal Medicine, Gifu University School of Medicine, Japan.
Biochem Biophys Res Commun. 1995 May 5;210(1):38-43. doi: 10.1006/bbrc.1995.1624.
Culturing hepatocytes on different extracellular matrix (ECM) substrata including tissue culture plastic, type I collagen, Engelbreth-Holm-Swarm (EHS) gel and poly-N-p-vinylbenzyl-D-lactonamide (PVLA) regulated levels of mRNAs for cytoskeleton and liver-specific genes. In hepatocytes on EHS gel, the ratio of albumin/beta-actin in mRNA levels was high and serially increased during the culture period, while the ratio was low and declined in cells on plastic substratum, collagen or PVLA. The changes in cellular levels of albumin mRNA which were regulated by ECM corresponded with those in two liver-specific transcription factors, hepatocyte nuclear factors-1 and -4, which control the transcription of liver-specific genes. These results suggest that cell-matrix interaction may determine and maintain the differentiated phenotype of hepatocytes by regulating liver-specific transcription factors.
在不同的细胞外基质（ECM）底物上培养肝细胞，这些底物包括组织培养塑料、I型胶原蛋白、恩格尔布雷特-霍尔姆-斯旺（EHS）凝胶和聚-N-对乙烯基苄基-D-内酰胺（PVLA），可调节细胞骨架和肝脏特异性基因的mRNA水平。在EHS凝胶上培养的肝细胞中，mRNA水平上白蛋白/β-肌动蛋白的比例较高，且在培养期间持续增加，而在塑料底物、胶原蛋白或PVLA上的细胞中，该比例较低且下降。由ECM调节的白蛋白mRNA细胞水平变化与两种肝脏特异性转录因子，即肝细胞核因子-1和-4的变化相对应，这两种转录因子控制肝脏特异性基因的转录。这些结果表明，细胞-基质相互作用可能通过调节肝脏特异性转录因子来决定和维持肝细胞的分化表型。
